The Microscopy Imaging Center (MIC), is a Larner College of Medicine Core Facility designed as a multi-user resource for the collection and analysis of biological images for clinical and research applications. The MIC consists of 11 microscopy-based imaging systems. At the light and electron microscopic levels, it provides state of the art, quality assured, morphologically oriented services. The MIC is operated on a fee for service basis and provides professional consultation and assistance with equipment use, experimental design and interpretation. We are located in Room 150 of the Firestone Medical Research Building.
Please use the following link to view our website: http://www.med.uvm.edu/mic
Check out this CBSR instructional and navigation-based video of how to work with the cores using iLab, https://youtu.be/YlKR2pny8Lc
MIC Safety Policy:
MIC requires that all users submit their Risk Management safety training transcript and CITI trainings as a PDF or a screenshot before using the MIC core independently. Please submit transcripts by attaching them to a New User Request form or MIC Biosafety Assessment form in iLab (detailed instructions below). Access to equipment schedules in iLab will not be granted until safety training transcripts are received.
Trainings #1-6 are required for all UVM lab workers.
Training #7 is required yearly.
Trainings #9-11 are dependent on your lab’s BSL designation and the work/samples you are bringing into the MIC & CBSR.
Training #8 is required for use of any laser-based microscope.
Course |
Type |
Requirement |
1. Emergency response for lab workers |
classroom training |
all UVM lab workers - once |
2. Keeping lab safe |
classroom training |
all UVM lab workers - once |
3. Laboratory Safety Roles and Responsibilities |
online through Risk Management |
all UVM lab workers - once |
4. Chemical Safety in the Laboratory |
online through Risk Management |
all UVM lab workers - once |
5. Laboratory Chemical Waste Disposal |
online through Risk Management |
all UVM lab workers - once |
6. Laboratory Ventilation and Chemical Fume Hoods |
online through Risk Management |
all UVM lab workers - once |
7. Annual Refresher Training 2023 |
online through Risk Management |
all UVM lab workers - annually after year of hire |
8. Laser Safety |
online through Risk Management |
Required for Nikon, Zeiss or Yokogawa confocals, STORM, AFM use |
9. CITI BSL (every 3yrs) |
online through CITI |
if you work in a BSL lab |
10. CITI OSHA BBP (annual) |
online through CITI |
Workers at risk for contact with human blood, tissues, primary cell lines or other potentially infectious material (OPIM) |
11. CITI Nanotechnology |
online through CITI |
if your lab works with nanotechnology |
https://www.uvm.edu/riskmanagement/train-and-inform-lab-personnel
Dr. Douglas Taatjes - Facility Director
In an ongoing effort to balance rigor and reproducibility as we strive to provide excellent customer service to all of our clients, if you experience any service issues please email Douglas.Taatjes@med.uvm.edu directly
Hours |
Location |
Normal Hours: Mon - Fri: 7:30am - 5:00pm
MIC Access (for trained users): 24/7 |
FMRB 150, 149 Beamount Ave |
Name | Role | Phone | Location | |
---|---|---|---|---|
Douglas J. Taatjes |
Facility Director
|
802-656-0373
|
douglas.taatjes@med.uvm.edu
|
FMRB 150
|
Nicole DeLance |
CBSR Business Manager
|
802-656-0813
|
nicole.delance@med.uvm.edu
|
FMRB 150
|
Amy Gilman |
CBSR Administrative Assistant
|
802-656-0813
|
amy.gilman@med.uvm.edu
|
FMRB 150
|
Nicole Bouffard |
802-656-0813
|
nicole.bouffard@med.uvm.edu
|
FMRB 150
|
|
Todd Clason |
802-656-0413
|
tclason@uvm.edu
|
Given E015
|
|
Kyra Lee |
802-656-0813
|
kyra.lee@med.uvm.edu
|
FMRB 150
|
|
Brad Vietje |
802-656-0813
|
brad.vietje@med.uvm.edu
|
FMRB 150
|
|
Jamie Levis |
802-656-0813
|
jamie.levis@med.uvm.edu
|
FMRB 150
|
Service Rates |
► 1. TEM (w/ Tech) (3) | |||
Name | Description | Price | |
---|---|---|---|
ImmunoGold exp (w/o antibody) | Inquire | ||
Lowicryl embedding |
1-2 specimens |
Inquire | |
Spurr's embedding |
First specimen |
Inquire | |
► 1. TEM (w/o Tech) (3) | |||
Name | Description | Price | |
ImmunoGold exp (w/o antibody) | Inquire | ||
Lowicryl embedding |
1-2 specimens |
Inquire | |
Spurr's embedding |
First specimen |
Inquire | |
► 2. SEM (w/ Tech) (5) | |||
Name | Description | Price | |
Carbon Evaporator | Inquire | ||
Critical point drying | Inquire | ||
EDS Instrument | Inquire | ||
SEM Processing |
SEM processing (fix & dehydrate) for first specimen |
Inquire | |
Sputter Coater | Inquire | ||
► 2. SEM (w/o Tech) (4) | |||
Name | Description | Price | |
Dry mounts | Inquire | ||
EDS Instrument | Inquire | ||
SEM Processing |
SEM processing (fix & dehydrate) for first specimen |
Inquire | |
Sputter coater | Inquire | ||
► 3. Light Microscopy (w/ Tech) (12) | |||
Name | Description | Price | |
Deparaffinize / Dehydrate Slides (1-10 slides) | Inquire | ||
EVG - Elastin Verhoeff-van Gieson (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
EVG-Trichrome (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
H & E staining (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
Immuno-Fluorescence Frozen Incubation (1-10 slides) | |||
Immuno-Fluorescence Paraffin Incubation (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
Immuno-Peroxidase Frozen Incubation (1-10 slides) |
Rate includes dehydration on second day of incubation. |
Inquire | |
Immuno-Peroxidase Paraffin Incubation (1-10 slides) |
Rate includes deparaffinization and dehydration on second day of incubation. |
Inquire | |
Oil Red O staining (1-10 slides) | Inquire | ||
PAS stain (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
Picrosirus Red staining (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
Trichrome staining (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
► 3. Light Microscopy (w/o Tech) (9) | |||
Name | Description | Price | |
Deparaffinize / Dehydrate Slides (1-10 slides) | Inquire | ||
EVG - Elastin Verhoeff-van Gieson (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
EVG-Trichrome (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
H & E staining (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
Immuno-Fluorescence Frozen Incubation (1-10 slides) |
UVM Internal
$61.46
each
|
||
Immuno-Fluorescence Paraffin Incubation (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
Immuno-Peroxidase Paraffin Incubation (1-10 slides) |
Rate includes deparaffinization and dehydration on second day of incubation. |
Inquire | |
Picrosirius Red Staining (1-10 Slides) |
Rate includes deparaffinization. |
Inquire | |
Trichrome staining (1-10 slides) |
Rate includes deparaffinization. |
Inquire | |
► 5. AFM (1) | |||
Name | Description | Price | |
Mica substrate | Inquire | ||
► 10. Leica BOND RXM auto-stainer (4) | |||
Name | Description | Price | |
3plex Calibration Slide | Inquire | ||
Multiplex Incubation |
Multiplex Incubation Charge (1-10 slides) Fee associated with an IHC or IF experiment on the BOND auto-stainer with multiple antibodies (provided by the user). |
Inquire | |
RNAscope Incubation |
RNAscope incubation charge (1-10 slides) Fee associated with an RNAscope run on the autostainer (RNAscope reagents and antibodies provided by user). |
Inquire | |
Single Plex Incubation |
Single Plex Incubation charge (1-10 slides) Fee associated with an IHC or IF incubation on the BOND with a single antibody (provided by the user). |
Inquire | |
► 11. Phenocycler (5) | |||
Name | Description | Price | |
Fusion Imaging | Inquire | ||
Phenocycler Conjugation - MIC reagents (1 antibodies) |
MIC will provide all required reagents to conjugate one antibody. |
Inquire | |
Phenocycler Conjugation - user purchased reagents (1-8 antibodies) |
User supplies the reagents required for the staining. MIC will provide a list of required reagents. Kits have reagents to conjugate 8 antibodies. |
Inquire | |
Phenocycler Staining - MIC reagents (1-4 slides) |
MIC will provide all required reagents to stain slides. |
Inquire | |
Phenocycler Staining - user purchased reagents (1-4 slides) |
User supplies the reagents required for the staining. MIC will provide a list of required reagents. |
Inquire | |
► 12. NanoString (1) | |||
Name | Description | Price | |
GeoMx nanoString Equipment Fee |
Digital Spatial Profiler (DSP) - Equipment Use. Billed per experiment. |
Inquire | |
► Technical Assistance (1) | |||
Name | Description | Price | |
Technical Assistance | Inquire | ||
► IVIS system (1) | |||
Name | Description | Price | |
IVIS Lumina whole animal imaging system (w/o tech) | Inquire |